This line generally yielded two PCR products of similar size and identical sequence from the fusion junction. This is currently being sorted out.
Picks up Hat7in Genbank searches. This is a mistake, the Hat7 sequence in Genbank is a chimera.
Will pick up homology to photosystem II protein X precursor in Gen bank searches. This annotation for the Arabidopsis sequence is not well supported by sequence similarity. Therefore, we have termed this a novel sequence.
The KS residues in the junction sequence are the -1 and -2 aa’s of EGFP, the ala10 linker is missing entirely. The hypothetical protein reading frame is in frame with GFP despite this odd junction. The A5A primer does not amplify this clone.