pEZT expression vectors - info
The sequences and maps presented here were assembled by piecing together bits of sequence from a variety of sources. The overall structure of the plasmids is correct, but we can’t vouch for the entire sequence. Here are some other important points about the sequences:
  • There are two junction areas for which no good sequence could be found. These short (8–12 bp) stretches of sequence are represented on the sequence maps by a string of 9-10 A-T base pairs and the label ‘unknown seq’.

  • The region flanked by the t-DNA borders in the pEZT vectors was cloned non-directionally into the pBBR1MCS-5 backbone (on a Sac I fragment), and I haven’t determined which orientation it is. The direction shown in the maps was chosen arbitrarily.
The following plasmids were used in construction of these vectors:
  • pEGFP-N1/pEGFP-C1 EGFP sequence and the MCS


    see CLONTECH Web Site for complete sequence info

  • pART7 35S promoter and ocs 3’ sequence

    Bart Janssen/Brett Morris
    Brett Morris
    Private Bag 92169
    Auckland, New Zealand

  • pBBR1MCS-5 broad host range plasmid

    Michael E. Kovach
    School of Medicine in Shreveport
    Louisiana State University Medical Center
    1501 Kings Highway
    PO Box 33932
    Shreveport, LA 71130-3932

    Kovach, M., et. al. (1995) Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175-6.

  • pDHB321.1 Basta cassette and T-DNA ends

    David Bouchez
    Laboratoire de Biologie Cellulaire
    INRA-Centre de Versailles F-78926 Versailles Cedex FRANCE

    Some common Agrobacterium strains used for transformation are Gm resistant (i.e. GV3101).

    Use Gm at 15 µg/ml. The low copy plasmid will not provide adequate resistence to 50 µg/ml.

David Ehrhardt and Patrick Eiken
Carnegie Intitution of Washington

Equipment and Protocols | Subcellular tag screen | 3D Imaging
Organelle dynamics 
| Cytokinesis | Dual FP imaging | Links
| Carnegie Plant Biology | Home

Copyright Sean Cutler and David Ehrhardt, all rights reserved.
Site designed and maintained by David Ehrhardt

We welcome feedback on the design and content of this site!