|Equipment and protocols|
Spinning Disk 2
Spinning disk confocal microscope with FLIM detection, laser scanning photomanipulation, and millisecond spherical abberation detection.
Configured by Intelligent Imaging Innovations, this microscope is our top instrument for imaging of molecular complexes in living cells.
Crystal Technology AOTF
Galvometer port switcher for confocal, FRAP or FLIM laser launch
63x Plan Apo 1.4 na oil
63x Plan Apo 1.3 na glycerin 21°C
63x Plan Apo 1.2 na water
20x Plan Apo 0.7 na imm
Photometrics Evolve 512x512 back-thinned EMCCD
ASI motorized stage with MCL piezo Z
Chroma ET 480/40
Chroma ET 525/50
Chroma ET 560/55
Chroma ET 595/50
Frequency domain fluorescence lifetime imaging
Pockel's cell excitation modulation
Lambert gated intensifier for emmision modulation
Photometrics CoolSnap CCD camera
Dual galvometer scanner with galvometer path switcher for bleaching and activation of arbitrary regions and rapid switching between photomanipulation and imaging.
A movable lens in the imaging path allows for spherical abberation correction. Part of the path switcher, the lens is positioned by a voice coil for rapid positioning so that correction can be performed throughout a rapid z-series.